Observations on the alkaline haematin/detergent complex proposed for measuring haemoglobin concentration.
نویسندگان
چکیده
The "alkaline haematin D-575" method for determining haemoglobin, described by Zander et al. [1984) Clin. Chim. Acta 136, 83-93) was tested. Claims that different non-ionic detergents in the reagent result in identical values for the haemoglobin concentration being measured could not be verified. It could also not be verified that a stable end-product with unique spectral characteristics is always reached within approximately 2 min and that conversion of fetal haemoglobin is faster than that with the haemiglobin cyanide method. Because of the many questions regarding the nature and characteristics of the alkaline haematin/detergent complex or complexes, it is not yet possible to recommend this method for routine haemoglobinometry.
منابع مشابه
Observations on the binding of haemoglobin and haematin by serum proteins in the rabbit. rat and guinea pig.
When haemoglobin is added to human plasma, it is bound through the globin portion of the molecule to an C2-globulin (Jayle, Boussier & Badin, 1952; Nyman, 1959). It has also been suggested that in human sera haemoglobin combines with albumin (Neale, Aber & Northam, 1958) and with a Plglobulin (Neale et al. 1958; Tombs, 1960), but these complexes could not be found by Allison & ap Rees (1957), A...
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In many health facilities in east Africa, haemoglobin estimation is performed using visual colour comparison methods. Efforts to establish colorimetric methods face numerous constraints, including the unavailability of standards for quality control. In contrast, the alkaline haematin D-575 method for haemoglobin estimation is a colorimetric method that uses primary standards prepared from pure,...
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In many health facilities in east Africa, haemoglobin estimation is performed using visual colour comparison methods. Efforts to establish colorimetric methods face numerous constraints, including the unavailability of standards for quality control. In contrast, the alkaline haematin D-575 method for haemoglobin estimation is a colorimetric method that uses primary standards prepared from pure,...
متن کاملInternational collaborative assessment study of the AHD575 method for the measurement of blood haemoglobin.
Accurate, economical methods for haemoglobin determination by laboratories in countries with limited resources are not available. This report provides the results of an international collaborative study evaluating the alkaline haematin detergent (AHD575) method as a reference method for laboratory services with limited resources. The study included 6 laboratories; 3 in East Mediterranean countr...
متن کاملDifferential effect of detergents on the alkaline denaturation of haemoglobin in maternal and fetal blood, with particular reference to Triton X-100.
In this investigation, the alkaline denaturation of haemoglobin in the blood of pregnant women and in cord blood obtained from newborn infants was followed by measuring the increase in absorbance at 375 nm. As expected, in the absence of detergent, the haemoglobin of cord blood was much more resistant to alkaline denaturation than that of maternal blood. However, in the presence of Triton X-100...
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عنوان ژورنال:
- Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie
دوره 27 4 شماره
صفحات -
تاریخ انتشار 1989